Police presence has been boosted in and around the Camp Nou stadium where Barça will play Roma and the Palau Sant Jordi auditorium where Madonna is performing in concert.
Football fans have been warned to arrive in plenty of time at the 90,000 seat stadium and to expect extra checks to enter. ID cards may be requested by police and backpacks have been banned.
"FC Barcelona and the Catalan police force, the Mossos d'Esquadra, have established a set of extraordinary security measures to increase the safety of those in attendance," said a statement from the club which advised fans to arrive up to three hours before kick-off.
The tightened security measures come in the wake of the Paris attacks in which 130 people died. Spain has remained on terror alert level four (out of a maximum five) following the attacks on November 13th.
Saturday saw "draconian measures" imposed ahead of El Clasico between Barça and Real Madrid at the Santiago Bernabeu stadium.
Football games - including the friendly match between Spain and Belgium last Monday – have been cancelled amid security fears and several high profile artists, including U2 and Prince have called off European concerts.
Some 30,000 people are expected to attend the sell-out Madonna concert in the first of two performances at the Palau.
The pop superstar vowed to continue with her Rebel Heart tour. "Why should I cancel? There is no one in the world who has the right to stop us from doing what we love," she said.