Travellers returning to Austria from mainland Spain must show a negative coronavirus test from Monday August 10th, Austria's foreign ministry said, or else undergo a 14-day mandatory quarantine.
Spain's Balearic and Canary islands will be excluded, it added.
Switzerland had already said on Wednesday it would add mainland Spain to its list of countries seen as having heightened risk of COVID-19 transmission and thus requiring travellers from there to undergo a quarantine.
Germany last week added three northern Spanish regions to its list of high-risk destinations, while France and Britain have also taken steps to limit travel from the country.
Austria already limits travel from numerous countries over virus fears, including fellow EU members Romania and Bulgaria, as well as several other Balkan states, including Serbia and Montenegro.