A radical movement of young separatists, Arran, called for a new demonstration “against repression” for 1600 GMT in central Barcelona.
The Catalan capital resembled a chaotic battleground Friday, the fifth consecutive day of protests over a Spanish court's jailing of nine separatist leaders on sedition charges over a failed independence bid two years ago.
Emergency services said Saturday that 152 people had been injured in overnight clashes in Barcelona, with dozens more hurt in the rest of Catalonia, taking the total to 182.
Authorities had already reported 500 injured since Monday even before the latest clashes erupted.
- Violent clashes break out in Barcelona on Friday night
- IN PICS: Barcelona protests turn violent
- Barcelona protests: General strike shuts down Catalonia
According to the interior ministry, 83 people were detained in the overnight violence, in addition to the 128 arrests police had reported before Friday's march.
“This can't go on, Barcelona does not deserve this,” said Ada Colau, the city's leftist mayor on Saturday, condemning “all kinds of violence”.
The air in the Catalan capital was still heavy with a burning stench as municipal workers cleared the streets of broken glass, rocks and rubber bullets, and repaired pavements where bricks had been ripped out.
“All this is very sad, and is not helping our cause,” said Assumpcio Segui, a 75-year old pro-independence pensioner.
Around half a million people had rallied in Barcelona on Friday in the biggest gathering since Monday's court ruling as separatists also called a general strike in the major tourist destination.
While most marchers appeared peaceful, hordes of young protesters went on the rampage near the police headquarters, igniting a huge blaze that sent plumes of black smoke into the air, as police fired teargas to disperse them, an AFP correspondent said.
Other fires raged near Plaza de Catalunya at the top of the tourist hotspot Las Ramblas, where hundreds of demonstrators rallied in defiance of the police, who tried to disperse them with water cannon. “Anti-fascist Catalonia!” they roared. “The streets will always be ours!”
Scores of police vans could be seen fanning out around the streets, their sirens screaming as the regional police warned people in a message in English on Twitter “not to approach” the city centre.
Earlier, many thousands of “freedom marchers”, who had set out to walk from five regional towns on Wednesday, arrived in Barcelona wearing walking boots and carrying hiking poles.
The rally coincided with the general strike, prompting the cancellation of flights, the closure of shops, business and several top tourist attractions, and slowing public transport to a trickle in a region that accounts for about
a fifth of Spain's economic output.
Activists also cut off Catalonia's main cross-border highway with France.
In downtown Barcelona, many shops and luxury outlets were closed on the city's Paseo de Gracia, with blackened, charred patches a testimony to the nightly clashes that have raged since Monday.
Manchester City's Catalan manager Pep Guardiola, an outspoken campaigner for the independence movement, urged European intervention to ease the crisis.
“The international community must help us to solve the conflict between Catalonia and Spain,” he said. “Some mediator from outside (must) help us sit (down) and talk.”
The Supreme Court's explosive decision has thrust the Catalan dispute to the heart of the political debate as Spain heads towards a fourth election in as many years, which will be held on November 10.