Manuel Agudo Durán (Nolito) scores the second goal against Spain on Friday. Photo: Bertrand Langlois/AFP
Vicente del Bosque's men bossed the Group D tie from the kickoff, with a double by Juventus' Alvaro Morata and a first competitive international goal from Nolito sealing a comfortable win.
“We showed our winning mentality and great team spirit here tonight,” said Andres Iniesta, who was awarded man-of-the-match.
“It depends on ourselves how far we go here, the feelings we have are very positive at the moment,” he said.
Turkey could also face UEFA sanctions after sections of their support lit flares and threw firecrackers onto the pitch at full-time.
Spain, aiming for an unprecedented third consecutive Euros title, can seal top spot in the pool with just a draw against Croatia in their final group game on Tuesday.
Turkey need to beat the Czech Republic in their final match and hope other results go their way to have any chance of progressing as one of the four best third-placed sides from the six groups.
“We have completed our first objective, to qualify for the next round, we controlled the game almost for the whole 90 minutes and we scored goals, so it was perfect,” Del Bosque said.
“But we haven't won anything yet, we are on a good path but there is still a long way to go,” he added.
Before the game Turkey coach Fatih Terim had called for more “fight” from his side to keep their Euro 2016 hopes alive after an out-of-sorts display against Croatia on Sunday, and for a while his players responded.
Sitting deep and launching the occasional counter-attack, Hakan Calhanoglu roused Turkey's raucous fans by sending a free-kick just over David de Gea's crossbar on 25 minutes.
From the half-hour though, Spain moved up a gear, starving Turkey of possession and, with Iniesta pulling the strings in midfield, carving out chance after chance.
And Terim lambasted his players for not giving their all for the cause.
“Today our national team threw in the towel, I didn't like it,” he said. “Spain are a special team, one of the strongest sides in the tournament, the first 30 minutes was ok but afterwards we didn't play well,” he said.
First Sergio Busquets teed up Manuel Agudo Durán [Nolito] for a right-footed curler just wide of left post. Then the busy Celta Vigo winger scuffed wide under pressure after a cutback from Juanfran.
On 34 minutes, though, Nolito turned provider to greater effect.
Looking up, he spotted Morata unmarked in front of goal and curled a pass over the defence to the marksman whose header gave Spain the lead. Three minutes later, Vicente del Bosque's men went two-up, this time with
Nolito as the finisher.
A chip into the box by Cesc Fabregas should not have reached its target but Mehmet Topal, off-balance, headed backward to the lurking Nolito who half-volleyed on the turn past Volkan Babacan.
After the break, despite some brief flurries of Turkish resistance, Spain quickly added a third on 48 minutes.
Iniesta, immaculate throughout, threaded a ball through the Turkish defence to Jordi Alba who squared to Morata for his second of the night, a tap-in through Babacan's legs.
It was all too easy after that for the two-time defending champions. Substitute Bruno Soriano nearly scored a fourth midway through the half, forcing a Babacan to tip round the post.
Then two minutes from the end he ballooned over when well-placed.
It was another desperately disappointing day for Turkey who had come into the tournament in fine form.
Individual mistakes caused at least one of the goals, said the 62-year-old Terim who guided Turkey to the European Championship semi-final in 2008.
“One of them was unbelievable, even the smallest chances you can't give Spain, otherwise there is no way to resist them”.
Fancied by some as dark horses to at least escape the group, they didn't manage a shot on target, while their fans even took to booing their star player Barcelona's Arda Turan late on.