Del Bosque will announce his final squad of 23 players to defend the trophy they won for the first time four years ago on May 25th, a day after Real Madrid and Atlético Madrid have disputed the Champions League final in Lisbon.
Manchester United's De Gea replaces the injured Victor Valdés as the third-choice goalkeeper, whilst Carvajal and Iturraspe are rewarded for their excellent seasons with Real Madrid and Athletic Bilbao respectively by offering cover in defence and midfield.
Nationalized Brazilian striker Diego Costa is included among the forwards after scoring 36 goals this season which have propelled Atlético to the brink of a Champions League and La Liga double.
Costa is one of four Atlético players included along with right-back Juanfran, midfielder Koke and Spain's all-time leading goalscorer at international level David Villa.
Chelsea's Fernando Torres is also included despite another difficult season at club level as are Juventus' Fernando Llorente and Alvaro Negredo of Manchester City with only one likely to make the final cut.
Real Madrid Álvaro Arbeloa has failed to make the grade, while Spain legend Carlos Puyol is not match fit.
Barcelona remain the most represented club with seven players from the Catalan club selected, including Andres Iniesta who scored the winner in the World Cup final back in 2010.
Four Real Madrid players are included in the list, while 13 are based outside Spain.
Iker Casillas (Real Madrid), Pepe Reina (Napoli/ITA), David de Gea (Manchester United/ENG)
Dani Carvajal, Sergio Ramos (both Real Madrid), Gerard Pique, Jordi Alba (both Barcelona), Cesar Azplicueta (Chelsea/ENG), Juanfran (Atletico Madrid), Javi Martinez (Bayern Munich/GER), Raul Albiol (Napoli/ITA), Alberto Moreno (Sevilla)
Sergio Busquets, Xavi Hernandez, Andres Iniesta, Cesc Fabregas (all Barcelona), Xabi Alonso (Real Madrid), Ander Iturraspe (Athletic Bilbao), David Silva (Manchester City/ENG), Santi Cazorla (Arsenal/ENG), Koke (Atletico Madrid), Tiago Alcantara (Bayern Munich/GER), Juan Mata (Manchester United/ENG)
Diego Costa, David Villa (both Atletico Madrid), Alvaro Negredo, Jesus Navas (both Manchester City), Pedro Rodriguez (Barcelona), Fernando Llorente (Juventus/ITA), Fernando Torres (Chelsea/ENG)